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KMID : 0613820180280080969
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Journal of Life Science
2018 Volume.28 No. 8 p.969 ~ p.976
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Establishment of Optimal Production Conditions of Transglucosidase Produced by Aspergillus niger
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Lee Jun-Yeob
Gang Seong-Ho
Kim Jong-Sik
Chung Chung-Wook
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Abstract
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In this study, transglucosidase (TG), an enzyme produced by Aspergillus niger, synthesized isomaltooligosaccharide from α-(1→4) linked substrates. The highest TG-producing A. niger KCTC6913 was selected from six kinds of species, and optimized TG producing conditions were established. Five different carbon sources (potato starch, sweet potato starch, corn starch, wheat starch, and dextrin) and three different nitrogen sources (yeast extract, malt extract, and beef extract) were tested to establish the carbon and nitrogen sources favorable for TG production. Measurements of TG activity after an initial culture at pH 5.0 for 15 days revealed that potato starch and yeast extract, which are basic culture media, resulted in the highest TG activity. In addition, A. niger KCTC6913 increased TG production under aerobic conditions and a controlled carbon/nitrogen ratio. In conclusion, to evaluate TG activity in the established optimal medium, it is confirmed that the basal and potato dextrose broth medium were used as a control, and the highest TG production was measured, which was highlighted in the established optimal medium.
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KEYWORD
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Aspergillus niger, optimal production condition, transglucosidase
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